The compounds measured with the methods are listed under each method. If the sample amount is very low and/or the matrix is new the detectability of the compounds of interest must be checked with a test sample before running the actual samples.
Results are delivered in an excel file. Fluxomics and 450+ metabolites methods are relatively quantitative and the results are given as peak areas. In all other quantitative methods the results are given in µM or other suitable concentration unit.
The raw files are also available if required.
Leftover samples are discarded unless otherwise agreed.
Raw data and all processing files are stored for 2 years after reporting.
Identification and within run relative quantitation of more than 470 metabolites using high resolution orbitrap mass spectrometer.
The exact number of detected metabolites is dependent on sample matrix and sample amount.
Link to comprehensive compound list.
Throughput approximately 150 samples per week.
Collaboration article DOI:10.1126/sciadv.adf7119, check publication list for additional publications.
Sample types (minimum amount)
Biofluids
Serum (110 µl)
Plasma (110 µl)
CSF (15 µl)
Sweat
Tears
Whole blood
Tissues (15 mg)
Mouse heart (0.5 mg)
Spleen (20 mg)
Brain
Muscle
Cecum
Gut
Skin
Mucus
Others
Feces
Cells (1x10e6)
Organoids
Spheres
Bacteria
Extracellular vesicles (10 µl)
Cell media
Quantitative LC-MS concentration measurement using internal standard with MRM
Link to comprehensive compound list.
Suitable for tissues, cells, and biofluids (https://doi.org/10.3390/metabo8030044, check publication list for additional publications).
Sample types (minimum amount)
Biofluids (110 µl)
Tissues (15 mg)
Cells (1x10e6)
Quantitative LC-MS concentration measurement using internal standard with triple quadrupole or high-resolution orbitrap mass spectrometer.
Compounds
Tetrahydrofolate
Dihydrofolate
5,10-methenyl tetrahydrofolate
5,10-methylene tetrahydrofolate
5-formyl tetrahydrofolate
5-methyl tetrahydrofolate
NOTE: Folate samples must be stored in amber coloured vials.
Sample types (minimum amount)
Whole blood (60 µl)
Tissues (20 mg)
Cells (1x10e6)
CSF (15 µl)
Quantitative LC-MS concentration measurement using internal standard with triple quadrupole or high-resolution orbitrap mass spectrometer.
https://doi.org/10.3390/metabo10030103
Compounds.
Glycine
Alanine
Serine
Aspartate
Glutamine
Glutamate
Pyruvate
Lactate
Fumarate
Succinate
Malate
Oxoglutarate
Cis-aconitate
Citrate
Isocitrate
Sample types (minimum amount)
Biofluids (110 µl)
Tissues (15 mg)
Cells (1.5x10e6)
Quantitative LC-MS concentration measurement using internal standard with triple quadrupole or high-resolution orbitrap mass spectrometer.
Compounds.
2-hydroxybutyric acid
3-hydroxybutyric acid
2-ketobutyric acid
3-ketobutyric acid (Acetoacetic acid)
Sample types (minimum amount)
Biofluids (110 µl)
Identification and absolute quantitation with internal standards using high resolution orbitrap mass spectrometer.
Throughput approximately 150 samples per week.
Compounds.
Acetic Acid
Propionic Acid
Butyric/Isobutyric Acid
Valeric Acid
Isovaleric Acid
2-Methylvaleric Acid
3-Methylvaleric Acid
Sample types (minimum amount)
Biofluids
Serum (110 µl)
Plasma
Whole blood
Tissues
Cecum (20 mg)
Others
Feces (10 mg)
Cells
Organoids
Spheres
Bacteria
Extracellular vesicles
Identification and absolute quantitation with internal standards using high resolution orbitrap mass spectrometer.
Throughput approximately 150 samples per week.
Compounds.
NADH - Reduced nicotinamide adenine dinucleotide
NAD - Nicotinamide adenosine dinucleotide
NR - Nicotinamide riboside
Me-4-py - N1-Methyl-4-pyridone-3-carboxamide
NADP - Nicotinamide adenine dinucleotide phosphate
NAAD - Nicotinic acid adenine dinucleotide
NAR - Nicotinic acid riboside
NMN - Nicotinamide ribotide
MeNam - N-methylnicotinamide
Me-2-py - N-methyl-2-pyridone-5-carboxamide
NADPH - Reduced nicotinamide adenine dinucleotide phosphate
Reduced prices are available if analyzing only NAD and NADH or NAD, NADH, NADP and NADPH.
Sample types (minimum amount)
Whole blood (60 µl)
Analysis of thousand lipid molecular species in a single method using the Lipidyzer platform.
More details doi: 10.1021/acs.analchem.1c02826
Sample types (minimum amount)
Biofluid (25 µl)
Tissues (15 mg)
Cells (1x10e6), Nanovesicles (25ul)
Isotope enrichment analysis and metabolic flux analysis of central carbon metabolism, glucose, NADolome, glutamine and other pathways using 13C-, 15N- and dH-tracers.
Collaboration article https://doi.org/10.1016/j.redox.2022.102416, check publication list for additional publications.
Requires consultation and collaboration.
Sample types (minimum amount)
Biofluids (10µl)
Tissues
Cells (30000)
Organoids
Spheres
Extracellular vesicles
Select metabolites from 450+ list. Direct orders via iLAB or for further information contact fimm-metabolomics@helsinki.fi.
We are open to develop via collaboration new metabolomics analytical methods both for precision metabolomics and research purposes. Contact the head of the unit.
We are open to develop via collaboration new metabolomics analytical methods both for precision metabolomics and research purposes. Contact the head of the unit.
As an example, these compounds have been quantitated from customer samples.
Riboflavin
GABA
L-Cystine
Pyruvate
Myo-inositol
Cortisol
Biotin
3,3',5'-Triiodothyronine
Glutathione
GSSG
We offer via collaboration help for metabolomics/lipidomics data analysis.
Contact the head of the unit.
We are open for consulting patient and clinical metabolomics work, teaching, seminar and conference talks.
Contact the head of the unit.