GBU performs Gateway cloning to various expression vectors, and site-directed mutagenesis of our ORF library clones.
We do cloning from the ORFeome collection, as well as from your own Gateway-compatible PCR products and plasmids to a number of destination vectors. Our selection of vectors includes a wide range of expression vectors ranging from bacterial and vertebrate expression to lentivectors. If your favourite vector is not on our list, feel free to suggest an update to our collection.
Additionally we provide help in designing primers to create Gateway-compatible PCR products from non-Gateway-compatible templates. We can then clone the PCR products to destination vectors in two cloning steps.
The cloned products are delivered as transfection grade minipreps and glycerol stocks. Minipreps can be delivered by mail if necessary.
You can make your cloning order with the same form as the library clone orders.
The following control constructs can be cloned to all destination vectors.
- EmGFP with stop
- EmGFP without stop
- mCherry with stop
- mCherry without stop
- lacZ with stop
- lacZ without stop
- empty insert (the destination vectors with this insert contain no ccdb gene and can be grown in DH5alpha and stbl3 competent cells)
|Customer class||University of Helsinki||Biocenter Finland||External|
|Cloning reaction||40 €||40 €||80 €|
first item in order
|Coming soon||Coming soon|
|Coming soon||Coming soon|
|Control construct||20 €||20 €||40 €|
Cloning of an ORFeome clone to an expression vector: processing fee of the clone: 20€, LR cloning to an expression vector: 40€, total 60€.
Cloning of your own PCR product to expression vector: PCR primer design free of charge, BP cloning to entry-vector: 40€, LR cloning to an expression vector: 40€, total 80€.
GBU also performs cloning reactions to customer-provided Gateway-compatible entry-constructs and PCR products. Please, deliver your samples to GBU sample fridge (Biomedicum Helsinki 1, 5th floor, room B505b). If you send samples by mail please use the address below:
Genome Biology Unit , 5th floor, rooms B505b ja B502a
Biomedicum Helsinki 1
00014 University of Helsinki
If you use a clone from the GBU in your publication, please acknowledge us in the following way:
Cloning and mutagenesis:
“Constructs were generated by the Genome Biology Unit supported by HiLIFE and the Faculty of Medicine, University of Helsinki, and Biocenter Finland"
“Name of the clone (ORFeome Library; Genome Biology Unit supported by HiLIFE and the Faculty of Medicine, University of Helsinki, and Biocenter Finland)"
“Name of the clone (MGC Library; Genome Biology Unit supported by HiLIFE and the Faculty of Medicine, University of Helsinki, and Biocenter Finland)"