Tagmentation-assisted multiplex pcr enrichment sequencing

Application: A tool for cervical cancer diagnostics and studying HPV evolution and adaptibility.
Unmet need: Traditional HPV test has poor clinical specificity for young individuals.
IP Status: Patent pending.
Keywords: Human papillomavirus (HPV), HPV genomic variability, HPV chromosomal integration,
cervical cancer, HPV test.

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Human papillomavirus (HPV) is the main cause of cervical cancer, which is one of the most common cancers in women worldwide Current HPV analysis methods are limited to either analysis of HPV variation or chromosomal integration TaME seq (tagmentation assisted multiplex PCR enrichment sequencing) is an innovative and cost effective sequencing approach, which combines tagmentation and multiplex PCR enrichment for simultaneous analysis of HPV variation and chromosomal integration, and it can also be adapted to other viruses Thus, it provides higher clinical specificity than any other HPV test on the market.

Sonja Lagström
Akershus University Hospital, Cancer Registry of Norway

Pekka Ellonen
University of Helsinki, Institute for Molecular Medicine Finland (FIMM)

Lagström, S. et al., 2019. TaME seq : An efficient sequencing approach for characterisation of HPV genomic variability and chromosomal integration. Scientific Reports, 9: 524.