Invention: A novel degron tag and auxin-perceptive protein to achieve rapid protein degradation.
Project status: Validated for nuclear, cytosolic and membrane-bound proteins.
IP status: Patent pending
Key words: Genome editing, degron tag, protein depletion, small-molecule induced.
Auxin-inducible depletion (AID) technology is a widely used method for rapid protein removal. However, current methods suffer from inefficient or premature degradation of target proteins and have thus limited their use. We have developed a highly potent AID system composed of a novel auxin perceptive protein and a novel degron tag. The system shows minimal basal degradation and enables rapid auxin-inducible depletion of mammalian proteins within 1 hour.
Lipid Trafficking Lab, University of Helsinki