Piloting multisensor imaging at NaPPI facilities

With the support from ViPS, NaPPI facility will offer free access to ViPS PIs to perform pilot experiments on "Brave New Initiatives" in the two whole-plant imaging facilities during the time period of December 2020- July 2021.

The two NaPPI facilities will be made available for UH ViPS PIs to bring in plants for imaging. Limited time series of imaging of plants with or without (pre)treatments will be organized. NaPPI personnel will provide guidance and materials for starting the experiment, program the plant management and imaging events, and assist in curating the imaging data. The users will obtain both image data from 1-to-3 sensors as well as the numeric data of plant size, morphology and physiological status at the chosen time points. 

To evaluate morphological and physiological plant phenotypes non-invasively over the course of time, multiple visible light RGBs, a thermal imaging set-up and two chlorophyll fluorescence imaging units (Imaging PAMs) are utilized. The UH installation has features for small plants (Compact system) as well as for large plant (Modular system) analysis. LED illumination is adjustable with smooth spectrum for given day length and light intensities, and plants can be light- or dark adapted in acclimation tunnels. These approaches allow measuring many structural and physiological parameters of samples from tiny Arabidopsis seedlings to large crops of over one-meter height. The high throughput facilities, with automated plant management (movement, weighing, watering), for large plants are in a greenhouse with environmental monitoring (relative humidity, temperature, light sensitivity and spectral quality) and for small plants in a controlled walk-in chamber. Analyzed plants till now include but are not limited to; Arabidopsis, herbs, berries, lettuce, potato, sweet potato, oil-seed Brassica, wheat, oat, spruce, birch and pine.

If you are UH ViPS PI and have an idea for a Brave New Initiative, please use the e-form below to apply for the access.