Assembly of an OCAA collection Entry vector (pENTR221)

If you ever wanted to know how to assemble the complete DNA sequence of a OCAA collection Entry vector, keep on reading...

Already a few times, students of mine did get stuck when they needed to assemble the full DNA sequence of an Entry vector from the ORFeome Collaboration (OCAA collection). We are lucky to have the Genome Biology Unit, which is a local distributor of OCAA clones. Although the parental vector sequence and the insert sequences are known, you still need to know exactly how to stitch them together. If you want to know how you can do it, I have been writing instructions on my blog: