CryoEM services offers full-service and self-service options for CryoEM work. Full-service option covers both sample preparation and imaging. Self-service customers will submit ready-made grids to our facility and imaging will be taken care of by our staff. Prices for both categories are given below.
All prices are VAT-exclusive. Standard rate of VAT is 24%. This price list is only for academic users. Prices for commercial customers are available on request.
Billing is three times a year.
FULL-SERVICE
Including Sample preparation and data collection as service.
Sample Preparation by Plunge freezing technique
- Standard grid 99,90 €
- Sample preparation service includes preparation of 3 parallel cryo-grids (Quantifoil Cu R1.2/1.3 grids and consumables) made by cryo-EM-staff. The grids will be stored for 6 months and they will be discarded without further notification.
- If other types of grids are requested and are available, the price will be obtained on request.
- Price includes: Preparation of EM-samples by plunge freezing technique, storage of cryo-sample for 6 months, consumables.
Negative staining
- This technique is used for sample optimization at room temperature. Cryo-EM-unit provides this method only as an additional service for optimisation of samples for cryo-EM. Price is available on request. The EM unit (Jokitalo) provides negative staining for non-cryo related projects as a regular service.
Data collection with Talos Arctica
Quick-check: 220 €/sample
- Used for sample optimization. Data set of the sample is typically 20 - 150 images. Such a data set will give you general information about your sample such as purity, size distribution and concentration.
- Price includes: Instrument time (Talos Arctica), operator and consumables. Sample preparation is not included.
Sample characterization: 440€/sample
- Representative data set of images. Data set of the sample is typically 100 - 300 images.
- Price includes: Instrument time (Talos Arctica), operator and consumables. Sample preparation is not included.
Large scale data collection for cryo-sample
- Large enough data set for particle reconstruction.
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24h, 1016 €. Data set of the sample is typically 500 - 1000 images.
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72h, 2744 €. Data set of the sample is typically 1000 - 3000 images.
- Price includes: Instrument time (Talos Arctica), operator and consumables. Sample preparation is not included.
Tomography (cryo)
Instrument time
- Talos Arctica 8 am – 4 pm 55 € / h, 4 pm – 8 am 36 € / h
- Leica EM GP 8 € / shift
- Glow discharge 3,30 € / use
Grids
- Quantifoil R2/2 Au Finder 6,00 €
- Quantifoil R2/2 Au 200Mesh 7,70 €
- Ultrathin carbon film on holey carbon 5,20 €
- C-flat 8,10 €
- UltraAuFoil R2/2 7,50 €
Consumables
- Autogrid 12,70 €
- Autogrid Container 20,65 €
- Operator time 39,00 €
SELF-SERVICE
Customer submit cryo grids, no sample preparation service included.
Sample preparation
- Leica EM GP 8 € / h, minimum booking time is 2h. Price includes blotting paper, ethane and LN2.
- Glow discharge 3,30 € / use
Purchase of grids and grid containers is customer’s responsibility. If the grid containersupplied by the customer is not suitable for autogrids, grids will not be saved after data collection.
Storage and book keeping of cryogrids is the customer’s responsibility. Sample sheet needs to be filled in for all samples/grids that are stored in CryoEM facilities or submitted to CryoEM service for imaging.
DATA COLLECTION, Talos Arctica
Quick-check:
Used for sample optimization. Data set of the sample is typically 20 - 150 images. Such a data set will give you general information about your sample such as purity, size distribution and concentration.
- Instrument time 220 €/sample(1
- Autogrid consumables 38 €/sample(1, 2, 3
Sample includes max 3 identical replica grids (identical concentration, grid type, sample preparation parameters etc.) and data will be collected from first suitable grid. Other grids will not be checked.
Sample characterization:
Representative data set of images. Data set of the sample is typically 100 - 300 images.
- Instrument time 440 €/sample(1
- Autogrid consumables 38 €/sample(1, 2, 3
Sample includes max 3 identical replica grids and data will be collected from first suitable grid. Other grids will not be checked.
Large scale data collection for cryo-sample
Typically large enough data set for particle reconstruction.
- Instrument time 1016 € / 24h Data set of the sample is typically 500 - 1000 images.
- Instrument time 2744 € / 72h Data set of the sample is typically 1000 - 3000 images.
- Autogrid consumables 12,70 €/grid (If used)
Cryo electron tomography
In tomography techique the specimen is tilted in the microscope to produce image series. Each image of the series is from same location and the images provide different orientations of the particles.
- Instrument time 1016 €
- Sample preparation not included
- Autogrid consumables 12,70 €/grid (If used)
MICROSCOPY CONSUMABLES
- Autogrid consumables 38 €/sample(1, 2, 3
- Autogrid container 22€/sample(4
- Sample includes max 3 identical replica grids (identical concentration, grid type, sample preparation conditions etc.)
- If customer submits autogrids, autogrid fee will not be charged
- If sample is loaded into the microscope, but it is not imaged, Autogrid fee will be charged.
- We can provide autogrid containers only if we have extra containers in storage.