In vivo Electrophysiology

Neuroscience Center provides cutting-edge approaches for in vivo electrophysiological recordings combined with optogenetics and behavioral readouts. Recordings can be performed in anaesthetized, awake head-fixed and freely moving mice. The head-fixed recordings can be combined with simple behavioral tasks using an air-lifted floating platform (Neurotar Mobile HomeCage).  

This system allows recordings of local field potentials (LFP) and multi-unit activity, to study for example network oscillations, synchronization and/or neural responses evoked by experimental event. Single unit activity (firing patterns of individual neurons) can be resolved after offline spike sorting. Recordings can be performed in anaesthetized or head-fixed mice using Mobile HomeCage. Optogenetics, pharmacological treatments and simple behavioral tasks can be combined.

Device : NeuroNexus Smartbox, with maximum of 256 channels from four silicon probes.

This setup supports high-resolution electrophysiological recordings in awake head-fixed mice running on a treadmill. Recordings are done using Neuropixel probes each allowing recordings from 384 cites. Simultaneous recordings with two 256-channel ECoGs can be combined for human-rodent translational research. Recordings can be combined with a visual task. 

Device Neuropixels 1.0 with PXIe acquisition module