SERVICES AND PRICES

BVC_prices

Our services include

  • Registration & Biosafety Training
  • BVC facility and laminar hood use
  • General BVC facility supervision and waste management
  • Replicating competent virus (RCV) test
  • Virus titer test for lentiviral particles (p24 test)
  • Lentivirus production
  • Retrovirus production
  • Sucrose purification
  • General cell lines related to virus work
  • Customer support
 

 

Biosafety training and registration

Biosafety training and registration are required when you wish to work in the BVC facility. Please contact our customer support to schedule the training. The biosafety training takes roughly 1-1.5h during which we go through the most significant biosafety issues and take a quick tour in our facility.

Please note that the biosafety training is only about how to use the facility safely. We do not provide protocols or guidance for the actual virus work. If you’re new to virus work, we recommend organising your own supervisor or someone else to train you for the protocols and habits. The supervisor/teacher/trainer also need to be a registered BVC user.

 

BVC facility use

After a customer has been trained and registered as a BVC facility user (STEMM users included) they are able to book the laminar hood for their experiments. All the facility instruments are free to use without extra fees. BVC users are also free to use all the cell culture plastics and reagents found in the facility. STEMM users need to provide their own accessories, plastics and reagents. Ethanol, MQ water, Virkon, pipettes and pipette boys are free for everyone.

 

RCV & p24 tests

Recombinant retro- and lentiviruses are designed to perform only one cycle of infection. After the transduction, a segment of the transfer vector that encodes shRNA/miRNA or cDNA integrates into the host cell's genome and is expressed under the control of the viral promoter. The recombinant viruses do not carry genetic information for production of virus particles and, therefore, cannot replicate or produce new virus particles in the target cells. However, there is a small possibility that unusual recombination events lead to formation of replication competent viruses during the virus production. The presence of replicating competent virus (RCV) is excluded in lentivirus preparations with the RCV test.

RCV test can be performed to transduced cells to ensure that the cells are virus-free before transferring cells from a BSL-2 laboratory a BSL-1 facility.

A 24 kDa capsid protein (p24) is a major structural core component in the recombinant lentiviruses. We use a p24 specific enzyme-linked immunosorbent assay (ELISA) to determine the p24 lentivirus capsid protein concentration (pg/ml) from the cell culture supernatants containing lentivirus preparations. 

These tests are performed every other Wednesday and the test schedule can be found from the BVC Online Hood reservation calendar. For RCV test you need to provide us 1 ml of unfiltered cell culture media. The media sample should be collected from a transduced cells after at least three passages, and the media should be kept on the cells at least 24 hours. If you are collecting the RCV test from suspension cell culture, remember to pellet and wash your cells with PBS during each passaging. For p24 titer analysis 10 μl of viral supernatant is needed. Inform the customer service whether your lentivirus media is concentrated or not, as this effects the sample preparation and final results. Label samples with your initials and running numbers (EH1, EH2…EHn). If you are a registered BVC facility user, you can deliver your samples in the -20°C sample box at the BVC facility. You can also contact our customer service to arrange the sample delivery. Remember to fill the order form and send it to bvc-support@helsinki.fi in order to have your samples analysed!

Please note! The p24 and RCV tests are not suitable for retroviruses!

 

Lentivirus products

 

BVC_lentivirus_figure

 

We produce lentivirus particles from the Genome Biology Unit’s TRC1 shRNA library, and the Sanger CRISPR knockout gRNA library. These library products are produced and distributed by GBU. Customers are also welcomed to deliver their own lentiviral or retroviral constructs to HelVi-BVC for custom viral particle production.

The Genome Biology Unit oversees the TRC and CRIPR libraries. The clones are available as glycerol stocks, plates, DNA minipreps or midipreps. To inquire more about these libraries, clones and GBU services please contact bcm-gbu@helsinki.fi.

For more information about the TRC and CRISPR libraries, please visit the GBU web page.

Our lentivirus products:

 

BVC_lentivirus_products

 

  • MINI: total volume 1,5 ml (2x 750 ml)
  • MINI gene set:
    • 5 shRNA clones / gene, 5 x 1,5 ml (5 x 2 x 750 ml)
    • 2 gRNA clones / gene, 2 x 1,5 ml (2 x 2 x 750 ml)
  • MINI library control (only when ordering other lentivirus products)
  • MIDI: total volume 6 ml (8x 750 ml)
  • Concentrated lentivirus 180 ml (18x 10 ml)
  • Concentrated lentivirus 380 ml (38x 10 ml)

Each lentivirus production process includes p24 titer test to determine the quality and concentration of the lentivirus particles. In addition, we use an internal control sample to evaluate the virus quality.

Here are presented the expected p24 titer concentration (pg/ml) ranges between different lentivirus products:

BVC_lentivirus_scales

Please note: The p24 and internal control analyses are for lentiviruses only. For retroviruses, quality control tests depend on the vector backbone and type (ecotropic or amphotropic pseudotypes). Please contact bvc-support@helsinki.fi for more information. Unfortunately HelVi-BVC doesn’t currently offer titer tests for retrovirus.

When you’re providing your own plasmids for virus production, here’s how much DNA you need to provide:

BVC_plasmid_amount

 

Please contact us for a quote request! We are happy to help you find the viral product most suitable to your needs! 

 

Retrovirus products

HelVi-BVC also produces retrovirus particles from customer DNA.

Our retrovirus products:

  • MINI: total volume 1,5 ml (2x 750 ml)
  • MIDI: total volume 6 ml (8x 750 ml)

At the moment HelVi-BVC doesn’t provide titer or replicating competent virus testing for retrovirus.

 

Sucrose purification

If you need to concentrate and purify your lentivirus supernatant, we can do this for you in HelVi-BVC by ultracentrifugation.

The lentivirus supernatant is pelleted and purified in a sucrose medium by ultracentrifugation and then resuspended in the volume of interest.

The minimum volume of lentivirus media required for concentration is 37 ml.

For further information please contact our customer support: bvc-support@helsinki.fi.

 

Cell lines

We also provide cell lines for virus production: HEK293 FT, Phoenix Amphotropic, Phoenix Ecotropic and HeLa cells.

Please note! It is the user’s responsibility to test the cells for absence of mycoplasma. In addition, the passage numbers of the cells vary between 7-10+, and we can’t promise a cell line with low passage numbers (1-5).

 

Price list for University of Helsinki customers

The HelVi-BVC research infrastructure is supported by University of Helsinki (HiLIFE and Research Programs Unit) and Biocenter Finland. The Biomedicum Virus Core price list presented below. The prices are applied for three different customer groups:

  1. Internal customers from University of Helsinki.
  2. BF customers from Biocenter Finland Universities.
  3. External customers outside University of Helsinki or BF circle (e.g. companies, institutes and non-BF universities). For external customers 24% VAT is added to the prices listed below.

For further information please contact bvc-support@helsinki.fi.

Service

Internal customers

BF customers

External customers

RCV test

40 €

50 €

105 €

p24 test

42 €

52 €

107 €

Biosafety training and registration

100 €

124 €

232 €

Hood hour

20 €/h

25 €/h

29 €/h

Cell ampouls

10 €

12,40 €

14 €

Lentivirus MINI,

     

Individual from library*

116 € + plasmid 

144 € + plasmid 

456 € + plasmid

TRC1 gene set*

152 € + plasmid

189 € + plasmid

497 € + plasmid

gRNA KO gene set*

137 € + plasmid

170 € + plasmid

479 € + plasmid

Customer plasmid

116 €

144 €

456 €

Lentivirus MIDI

     

From library*

140 € + plasmid

170 € + plasmid

483 € + plasmid

Customer plasmid

140 €

170 €

483 €

Concentrated lentivirus,180 ul

     

From library*

186 € + plasmid

231 € + plasmid

668 € + plasmid

Customer plasmid

186 €

231 €

668 €

Concentrated lentivirus, 380 ul

     

From library*

243 € + plasmid

302 € + plasmid

778 € + plasmid

Customer plasmid

243 €

302 €

778 €

Retrovirus MINI

53 €

66 €

383 €

Retrovirus MIDI

92 €

114 €

428 €

Sucrose purification of concentrated lentivirus

35 €

43 €

187 €

*) The plasmids from TRC and CRISPR libraries are prepared and distributed by the GBU core. The plasmid preparation fees are charged separately and those are not included in the virus production prices presented above. For further information please contact GBU customer service or visit their web page.