The Biomedicum Stem Cell Center (BSCC) core facility is a comprehensive provider of human pluripotent stem cell services at the Meilahti campus of the University of Helsinki. iPSC-derived, disease specific cell models serve as an opportunity to study pathophysiological mechanisms and can lead to development of new treatments. We provide locally derived human pluripotent stem cell lines and generate induced pluripotent stem cell (iPSC) lines from somatic cells provided by the client. BSCC operates on a fee-for-service basis in providing custom iPSC derivation using the latest technologies. BSCC is a part of the life science infrastructure of HiLife GoEditStem platform and the Stem Cells and Biomaterials consortium, national Biocenter Finland technology platform.
BSCC is equipped with basic cell culture equipment such as laminars, incubators, centrifuges and microscopes. For further information, please visit https://www.helsinki.fi/en/researchgroups/pluripotency-and-disease-modeling/bscc-core-facility
Contact person: BSCC Director Timo Otonkoski, timo.otonkoski@helsinki.fi or BSCC Coordinator Ras Trokovic, ras.trokovic@helsinki.fi
Location: Biomedicum Helsinki, Biomedicum Stem Cell Center, 5th floor, Haartmaninkatu 8, Meilahti Campus
Services
- Cell distribution
- Derivation and characterization of iPSC lines
- User training and education
- Miscellaneous stem cell research services
- Genome editing, on demand
- Differentiation into endodermal lineages, on demand
Instruments
- Thermo Forma Water Jacketed CO2 Incubator (2 x)
- Heraeus Multifuge 3S-R (w/ tube and plate rotor)
- Grant waterbath
- Sartorius Biohit MidiPlus pipettors
- Stuart vortex
- Kojair Biowizard Biosafety cabinet
- 4D-Nucleofector™
- Eppendorf Centrifuge 5702
Applications
- Distribution of locally derived human pluripotent stem cell lines
- Non-integrative technologies for reprogramming of client provided somatic cells
- Customized training and education
User fees
The research infrastructure is supported by University of Helsinki (HiLIFE) and Biocenter Finland. The prices are listed below.
| Product | Volume | University of Helsinki price (VAT 0) eur | Academic price (not included VAT) eur | Non-academic price (not incl. VAT) eur |
|---|---|---|---|---|
| Use of Cell IQ | 1 plate/h | 2 | - | |
| Frozen hPSC | 1 ampoule | 120 | - | |
| Thawed hPSC | 1 plate | 200 | - | |
| Culture of hPSC | passage | 50 | - | |
| hiPSC generation from fibroblasts | 2 characterized clones, frozen at passage ten and 2-4 uncharacterized clones frozen at passage 3 | 2200 | 2983 | 3233 |
| hiPSC generation from blood | 2 characterized clones, frozen at passage ten and 2-4 uncharacterized clones frozen at passage 3 | 2800 | 3720 | 4005 |
| Early hiPSC generation from fibroblasts | 4-6 uncharacterized clones frozen at passage 3 | 1000 | ||
| Early hiPSC generation from blood | 4-6 uncharacterized clones frozen at passage 3 | 1800 | ||
| Fibroblast culture from skin biopsy for hiPSC generation | 300 | - | ||
| DNA Fingerprint analysis | 1 cell line | 120 | - | |
| Miscellaneous stem cell research services | 1 hour | 44 | 44 | 60 |
| Genome editing | Generation of knockouts within GoEditStem platform (HiLIFE) | On demand | ||
| Differentiation into endodermal lineages | On demand |