Sanger sequencing

FIMM Technology Centre's SeqLab provides DNA sequencing services for the scientific community nationally and internationally. In addition to DNA sequencing, small to medium scale fragment analysis service is also available. Sequencing system used is Applied Biosystems ABI3730XL DNA Analyzer. As a starting material we use purified PCR products mixed with sequencing primer or ready made sequencing/fragment analysis reactions. Maximum read length (QV>20) for high quality sequencing product is around 600bp.

For more detailed instructions please contact fimm-seqlab(at)helsinki.fi

SeqLab is currently transitioning to using HiLife iLab online system for managing orders and invoices. iLab has been in use for other sequencing services in the Sequencing Unit for some time now, and during 2021 we are planning to start using it also for capillary sequencing services. We encourage you to familiarize yourself with iLab and use it to place your upcoming sequencing orders! In iLab you will find the sample submission form along with information about sample processing and up to date pricing for available services.

If you need help with iLab the Seqlab staff is happy to help you get started! Please send email to fimm-seqlab(at)helsinki.fi for any feedback or questions. Below are instructions on how to register to iLab and make a work request for Sanger and fragment analysis services.

 

  • Optional BigDye v.3.1 sequencing reaction for customer's template-primer cocktails.
  • Sequencing reaction purification: dye terminator removal with Optima DTR 96-Well filter plates.
  • Electrophoresis with ABI3730xl DNA Analyzer.
  • Basecalling with Sequencing Analysis 5.2.
  • Results delivered via e-mail (.ab1 file format).