FIMM Technology Centre /

FIMM HIPREP

HiPrep core is a new service unit of the FIMM Technology Centre. It offers RNA isolation or preparation services for both academic and industry customers. HiPrep core develops workflows for preparation of high-quality nucleic acids from various biological samples in close collaboration with the FIMM sequencing and single cell analytics units and the EV Core.

New customers or projects

Please contact the HiPrep personnel

Sample types
Equipment
Personnel

Sample types

sample_type
  • PaxGene Blood RNA samples
  • PBMC (Peripheral blood mononuclear cells)
  • Serum, plasma and other cell free RNA (cfRNA) samples
  • Frozen tissue including tissue sections
  • Extracellular vesicles, see EV core
  • Other
  • Read about HiPrep´s participation in IBBL’s Biospecimen Proficiency Testing program as part of EATRIS-Plus
    • Tested and yield/purity/integrity -certified sample types: cell-free RNA (cfRNA) Extraction from Plasma, RNA Extraction from Whole Blood, RNA Extraction from formalin fixed paraffin embedded (FFPE) Tissue Material, and Total RNA Extraction from Frozen Tissue.

Equipment

Chemagic2 MaxWell_small Tapestation_small

Chemagic 360 (Perkin Elmer)

  • Nucleic acid isolation with magnetic beads
  • High-throughput processing up to 96 samples simultaneously

MaxWell RSC48 (Promega)

  • Nucleic acid isolation with magnetic beads
  • Medium throughput, processing up to 48 samples simultaneously
  • Uses Maxwell® RSC prefilled cartridges, which reduce variability and contamination risk in sample processing

TapeStation 4200 (Agilent Technologies)

  • Nucleic acid quality control
  • Capacity up to 96 samples per run
  • Measures DV200, RIN and DIN values, concentration estimation

Bravo NGS workstation (Agilent Technologies)

  • Liquid handling / sample preparation, e.g. proteinase K treatments, aliquots
  • High-throughput processing up to 96 samples simultaneously (installed in summer 2021)

Precellys 24 (Berting Techologies)

  • Tissue/cell homogenizer, beat beating
  • Capacity up to 24 samples per run