Biacore T100 can be used to monitor almost any molecular interactions as long as one of the interacting partner can be immobilized on a sensor surface. It characterizes molecules in terms of the specificity of interaction, on and off -rates (kinetics), binding strength (affinity) and thermodynamics. It can also be used for concentration measurements of active biomolecules to monitor the effects of purification or storage conditions on molecule preparations. Biacore utilizes label-free technology which can be used to study:
- protein interactions from small peptides to multiple sub-unit protein complexes
- membrane biochemistry or membrane-bound receptor interactions using native membranes, artificial membranes or vesicles
- replication, transcription and translation of nucleic acids, hybridization of DNA and RNA
- the specific recognition of cell surface carbohydrates and the effects of glycosylation on molecular interactions